Prof Philip Poole
Plant-Microbe interaction, Synthetic biology
Nitrogen (N) is one of the primary limiting nutrients for plant growth. The high demand for N fertilization in agriculture has led to environmental catastrophe in some parts of the world due to intensive application of inorganic N fertilizers, while in others, yields of cereal crops are limited by the economic inaccessibility of such fertilizers. Both of these issues could be circumvented by transferring the ability to acquire biologically fixed N to cereal crops, a trait that is currently mostly limited to the symbiosis between legumes and N-fixing rhizobia.
Using fundamental knowledge of nitrogen regulation in endophytic nitrogen fixing bacteria, we will engineer strains, which excrete ammonium to benefit crop growth, and examine the potential of these modified strains as inoculants.
Rodrigues AM, Mendes MD, Lima AS, Barbosa PM, Ascensão L, Barroso JG, Pedro LG, Mota MM, Figueiredo AC. 2017. Pinus halepensis, P. pinaster, P. pinea and P. sylvestris essential oils chemotypes and monoterpene hydrocarbon enantiomers, before and after inoculation with the pinewood nematode Bursaphelenchus xylophilus. Chem Biodivers 14(1):e1600153.
Mendes MD, Barroso JG, Oliveira MM, Trindade H. 2014. Identification and characterization of a second isogene encoding γ-terpinene synthase in Thymus caespititius. J Plant Physiol 171:1017-1027.
Mendes MD, Figueiredo AC, Oliveira MM, Trindade H. 2014. Influence of culture media and fungal extracts on essential oils composition and on terpene synthase gene expression in Thymus caespititius. Plant Cell Tiss Org 118(3):457-469.
Miguel MG, Nunes S, Cruz C, Duarte J, Antunes MD, Cavaco AM, Mendes MD, Lima AS, Pedro LG, Barroso JG, Figueiredo AC. 2013. Propolis volatiles characterization from acaricide treated and untreated beehives maintained at Algarve (Portugal). Nat. Prod Res 27(8):743-749.
Mendes MD, Figueiredo AC, Oliveira MM, Trindade H. 2013. Essential oil production in shoot cultures versus field- grown plants of Thymus caespititius. Plant Cell Tiss Org 113:341-351.
Barbosa P, Faria JMS, Mendes MD, Dias LS, Tinoco MT, Barroso JG, Pedro LG, Figueiredo AC, Mota M. 2012. Bioassays against pinewood nematode: assessment of a suitable dilution agent and screening for bioactive essential oils. Molecules 17(10):12312-12329.
Mendes MD, Lima AS, Trindade H, Correia AID, Barroso JG, Pedro LG, Figueiredo AC. 2011. ISSR molecular characterization and leaf volatiles analysis of Pittosporum undulatum Vent. naturalized in the Azores archipelago (Portugal). Ind. Crops Prod 33(3):710-719.
Dandlen SA, Lima AS, Mendes MD, Miguel MG, Faleiro ML, Sousa MJ, Pedro LG, Barroso JG, Figueiredo AC. 2011. Antimicrobial activity, cytotoxicity and intracellular growth inhibition of Portuguese Thymus essential oils. Rev Bras Farmacogn 21(6):1012-1024.
Faria JM, Sanches J, Lima AS, Mendes MD, Geraldes DA, Leiria R, Trindade HD, Pedro LG, Barroso JG, Figueiredo AC. 2010. Eucalyptus from mata experimental do Escaroupim (Portugal): evaluation of the essential oil composition from sixteen species. Proc. XXVIIIth IHC - IHC Seminar: A New Look at Medicinal and Aromatic Plants. Acta Hort (ISHS) 925:61-66.
Dandlen SA, Lima AS, Mendes MD, Miguel MG, Faleiro ML, Sousa MJ, Pedro LG, Barroso JG, Figueiredo AC. 2010. Antioxidant activity from six Portuguese thyme species essential oils. Flavour Frag J 25(3):150-155.
Mendes MD, Trindade H, Figueiredo AC, Pedro LG, Barroso JG. 2010. Trichomes morphology and essential oils composition in micropropagated vs. field grown Thymus caespititius plants. Acta Hort (ISHS) 860:215-218.
Lopes VR, Barata AM, Farias R, Mendes MD, Lima AS, Pedro LG, Barroso JG, Figueiredo AC. 2010. Morphological and essential oil variability from nine Portuguese fennel (Foeniculum vulgare Mill.) accessions. Acta Hort (ISHS) 860:33-49.
Mendes MD, Trindade H, Figueiredo AC, Pedro LG, Barroso JG. 2009. Chaerophyllum azoricum Trel. grown in the Azores archipelago (Portugal): Evaluation of the genetic diversity using molecular markers and comparison with volatile oils profiles. Flavour Frag J 24:259-265.
Mendes MD, Trindade H, Figueiredo AC, Barroso JG, Fontinha SS, Pedro LG. 2009. Volatile and molecular characterization of two Portuguese endemic species: Angelica lignescens and Melanoselinum decipiens. Biochem. Syst. Ecol. Biochem Syst Ecol 37:98-105.